Table 2. Mutations found in covS gene of GAS strain 5448/covS ⁺ during infection in mice.^a .

Mouse	Tissueb	Gene Mutation	NTc	# bpd	# clonese	Protein Mutationf
1	Spleen	Deletion	974–1140	167	1	Delete 325–380
	Spleen	Deletion	1243	1	2	Stop-458
2	Spleen	Deletion	1243	1	2	Stop-458
3	Spleen	Deletion	279–297	19	10	Stop-156
4	Spleen	Deletion	279–297	19	3	Stop-156
	Spleen	Insertion	96	11	2	Stop-39
	Spleen	Mutation	404–405, 845	3	2	S135F, L282S
5	Spleen	Insertion	468	1	7	Stop-165
6	Spleen	Insertion	1357	1	7	Stop-478
	Spleen	Deletion	83	1	3	Stop-36
7	Spleen	Deletion	229–840	612	2	Delete 77-280
	Skin	Deletion	229–840	612	4	Delete 77-280
8	Spleen	Deletion	229–840	612	6	Delete 77-280
	Skin	Deletion	229–840	612	3	Delete 77-280
9	Spleen	Deletion	229–840	612	5	Delete 77-280
	Skin	Deletion	229–840	612	1	Delete 77-280
10	Spleen	Deletion	229–840	612	5	Delete 77-280
	Skin	Deletion	229–840	612	1	Delete 77-280
11	Spleen	Deletion	229–840	612	2	Delete 77-280
12	Spleen	Deletion	1104–1140	37	6	Stop-377
	Skin	Insertion	140	31	1	Stop-71
13	Spleen	Deletion	229-840	612	1	Delete 77-280
	Spleen	Insertion	160	31	1	Stop-71
	Skin	Insertion	998	4	1	Stop-339
14	Spleen	Insertion	160	31	1	Stop-71
	Spleen	Deletion	1104–1140	37	1	Stop-377
	Skin	Deletion	1211–1215	5	1	Stop-407
15	Spleen	Deletion	1104–1140	37	1	Stop-377
	Skin	Insertion	96	11	1	Stop-39
16	Skin	Insertion	140	31	2	Stop-71
17	Skin	Deletion	1376–1382	7	1	Stop-466

^aPutative domains of CovS as defined elsewhere [8], [11], where mutations occur: CovS: TMI (22–33); EC Loop (34–180); TMII (181–205); HAMP (208–266); HisKA (269–333); HATPase (386–492).

^bTissue from which clones were obtained.

^cNucleotide (NT) position of change, or range of change, based on position of the covS start codon.

^dNumber of nucleotides of WT-covS replaced, inserted, or deleted.

^eNumber of clones sequenced.

^fEffect on CovS protein.