Figure 6. Nitrotyrosine levels are lower in the center of mycobacterial granulomas in zebrafish embryos.

(A) Example fluorescence confocal micrographs of anti-nitrotyrosine staining performed on 3 dpi granuloma structures in wild type embryos infected with Mm showing a single Z-plane. (B) Gray values of the bacterial and anti-nitrotyrosine fluorescence signals measured along a straight line through the center of the granuloma along the longest axis of the granuloma (see white dashed lines in (A)), intensity of the fluorescent signal was measured using ImageJ. Numbered peaks in the graphs correspond to numbered patches of tyrosine nitration in (A).