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. 2014 Jun 26;9(6):e100931. doi: 10.1371/journal.pone.0100931

Figure 1. Chronic thrombin treatment increases Cx43 protein expression.

Figure 1

HPAECs were left untreated or treated hourly with thrombin (0.5 U/mL) over 6 hr. and Cx43 protein and mRNA were measured. A.) Representative immunoblot shows Cx43 protein levels in thrombin (thr) or control (C) HPAECs. Panel images for control and thrombin bands were recombined from images generated from a single membrane of a Western blot. B.) Graph shows densitometric analysis of Cx43 protein expression. Cx43 protein levels were normalized to β-actin levels and divided by the average control value. *, p<0.05, n = 9. C.) Cx43 mRNA levels were measured by real-time RT-PCR and normalized to GAPDH mRNA and divided by the average control value. *p<0.05, n = 14.