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. 2014 Jun 26;9(6):e100931. doi: 10.1371/journal.pone.0100931

Figure 4. LPS and chronic thrombin treatments increase intercellular communication.

Figure 4

HPAECs were grown to confluence on glass cover slips and treated with LPS (200 ng/mL for 24 hr) or chronic thrombin (0.25 U/mL; once every hr for 6 hr.) Single cells were injected with Lucifer yellow for 1 min., and cells were photographed after an additional 5 min. A.) Photomicrographs show representative fluorescence images of an injected cell and its neighbors after injection of Lucifer yellow in a control culture or a culture treated with LPS. The injected cell is marked with a black arrow; neighboring cells which show dye transfer are marked with a white asterisk. B.) Graph shows the composite data for Lucifer yellow dye transfer in control or LPS-treated HPAECs. Values shown are normalized by dividing the number of dye-containing neighbors by the average value obtained by injections in control cultures. **, p<0.001. n = 4. C.) Graph shows the composite normalized data for Lucifer yellow dye transfer in control or thrombin-treated HPAECs. *, p<0.05, n = 3.