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. 2014 Jun 26;9(6):e100931. doi: 10.1371/journal.pone.0100931

Figure 6. Gap junction inhibitor carbenoxolone attenuates permeability response to thrombin.

Figure 6

Confluent HPAECs were treated with carbenoxolone (carb) for >5 min (red triangle; 100 µM) followed by treatment with thrombin (arrow; 0.5 U/mL) and transendothelial resistance (TER) was measured. TER was recorded prior to any treatments and continued an additional 1.5 hr after treatments. A.) Representative TER traces are shown. B.) Peak permeability response is given as [1–(minimum normalized TER)], where normalization is to TER at the time point just prior to the first treatment. **, p<0.001; *, p<0.05; n = 13 for control, n = 11 for carb alone, and n = 15 for thr alone and thr+carb. C.) Permeability response after recovery is given as (1–normalized TER), where TER is taken at the time point in recovery where the thrombin alone group recovers to reach a permeability of <0.25; this is roughly 1 hr after thrombin treatment. As before, TER is normalized to the time point just prior to the first treatment. *p<0.001, n = 13 for each group. Carb = carbenoxolone, Thr = thrombin.