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HPAECs were treated with carbenoxolone for >5 min followed by thrombin treatment (0.5 U/mL; 30 min) and collected for Western Blot. A.) Representative Western Blot is shown. B.) Phospho-MLC protein was normalized to β-actin relative to control. **, p<0.001. *, p<0.05. n = 5 for control (C), thrombin alone (thr), and thrombin+carbenoxolone (thr+carb) groups. n = 1 for carbenoxolone (carb).
