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. 2014 Jun 26;9(6):e100931. doi: 10.1371/journal.pone.0100931

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© 2014 O’Donnell et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Cultures of HPAECs were transfected with Cx43 or control (non-targeting) siRNA 48 hrs prior to various analyses. A.) Western Blots show levels of Cx43 in untreated HPAECs (C) or in cells transfected with different concentrations of non-targeting (NT) or Cx43 (si) siRNA. B.) Fluorescence microscope images show HPAECs micro-injected with Lucifer yellow after transfection with non-targeting (NT) or Cx43 (siRNA-Cx43) siRNA. C.) Graph shows the degree of dye transfer in cultures of HPAECs microinjected with Lucifer yellow after transfection with non-targeting (NT) or Cx43 (siRNA-Cx43) siRNA. Dye transfer was normalized by dividing the number of dye containing neighbors by the average value obtained in NT transfected controls. **, p<0.001, n = 6 for each group.