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. 2014 Jun 26;9(6):e100883. doi: 10.1371/journal.pone.0100883

Figure 2. GPBAR1 agonism reduces cytokine production induced by various TLR ligands.

Figure 2

(A) Production of cytokines at 24 hrs by LPS-stimulated monocytes (1 donor, quadruplicate) was assessed in the presence of EX00000246 (500 nM) or Lithocholic acid (10 µM) by BioRad FlexMap 3D technology using a multi-plex kit purchased from Millipore.Human monocytes (B) and monocyte-derived macrophages (C) (4 donors, assayed in triplicate) were stimulated with various TLR ligands in the presence of EX00000246 and production of IL-12p40 as measured by MSD was assessed at 24 hrs. Untreated negative controls reported cytokine levels at or below the detection limit and therefore are not plotted in these graphs.