Table 1. Antibodies.
| Primary antibodies | ||||
| Target | Host | Clonality | Dilution | Source |
| Insulin | Mouse | Monoclonal | 1∶1000 | Abcam, ab6995 |
| Glucagon | Guinea-pig | Polyclonal | 1∶200 | Raised in-house – PCA2/4 |
| GLP-1 | Rabbit | Polyclonal | 1∶200 | Raised in-house – XJIC8 |
| GIP | Rabbit | Polyclonal | 1∶400 | RIC34/111J, kindly donated by Professor L Morgan, Guildford, UK |
| Ki67 | Rabbit | Polyclonal | 1∶200 | Abcam, ab15580 |
| Secondary antibodies | ||||
| Target | Host | Reactivity | Dilution | Source |
| IgG | Goat | Guinea-pig | 1∶400 | Alexa Fluor 488, Invitrogen, UK |
| IgG | Goat | Mouse | 1∶400 | Alexa Fluor 594, Invitrogen, UK |
| IgG | Goat | Rabbit | 1∶400 | Alexa Fluor 488, Invitrogen, UK |
| IgG | Goat | Rabbit | 1∶400 | Alexa Fluor 594, Invitrogen, UK |