Skip to main content
. 2014 Jun 26;9(6):e101015. doi: 10.1371/journal.pone.0101015

Figure 1. Selection of ssDNA aptamers targeting DNA-bound cJun.

Figure 1

A) cJun was incubated with the immobilized DNA and the complexes were washed prior to the addition of the ssDNA pool. Six rounds of SELEX were performed with rounds 5 and 6 including a negative selection as illustrated by the dashed arrows. B) Four individual sequences from round 6 of the selection, the conserved motifs are highlighted. C) EMSAs with aptamer-1, 16, 19, and 27. For each aptamer, either cJun/cJun or cJun/cFos was titrated from 0.4 nM to 6.5 nM.