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. 2014 Jun 26;9(6):e101260. doi: 10.1371/journal.pone.0101260

Figure 2. Effect of free bisphosphonates on human macrophage cultures.

Figure 2

Count of adherent and non-adherent macrophages by Trypan Blue staining after 48 h of continuous treatment with four different bisphosphonates at concentrations ranging from 0.25 to 1.0 mM A). MTS evaluation of cell viability after 48 h and 6 days of continuous treatment with four different bisphosphonates at concentrations ranging from 0.25 to 1.0 mM B). Results are mean ± SD of at least 3 experiments performed in duplicate. CTL = controls; Clo = Clodronate; Zol = Zoledronate; Pam = Pamidronate; Iba = Ibandronate. *** p<0.001, **p<0.01 and *p<0.05 by ANOVA analysis with Bonferroni post-hoc test.