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. 2014 Jun 26;10(6):e1004165. doi: 10.1371/journal.ppat.1004165

Figure 7. Parasite-induced arg1 expression in macrophages is dependent on STAT6.

Figure 7

Expression of A) STAT6 mRNA and B) arg1 mRNA in BMDMs that were uninfected (Un) or infected in vitro with L. donovani (Inf) for 24 h after transfection with STAT6-specific knockdown siRNA (STAT6 KD) or scrambled siRNA (Control). Shown is the mean and SEM of the fold-change in mRNA compared to unstimulated controls as determined by qRT-PCR in 6 replicates from 2 independent experiments. C) Parasite burden at 24 h post-infection of STAT6 KD BMDMs or control. Shown is the mean and SEM of the parasite burden with reference to control (uninfected) cells in 4 replicates determined by qRT-PCR. D) STAT6 and arg1 mRNA expression in splenic macrophages from L. donovani infected hamsters 48 hrs after ex vivo transfection with STAT6-specific siRNA (STAT6 KD) or scrambled siRNA (Control). Data are shown as the mean and SEM of the percent of maximal mRNA expression in the control samples. *p<0.05; ***p<0.001.