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. 2014 May 28;15(1):406. doi: 10.1186/1471-2164-15-406

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© Sanders et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Diagram illustrating approach taken for adjusting for gametic expression. DE analysis was conducted between male and female gonozooid libraries (step 1), identifying significantly up- or down-regulated transcripts (step 2). Those transcripts found to be significant were excluded from the template pool and a DE analysis on those non-significant transcripts included counts from both male and female libraries (step 3 shown in black). Then, a second DE analysis was conducted on transcripts up-regulated in female gonozooids. For these comparisons, only the expression counts from male gonozooids libraries were used (step 3 shown in red). Results from the both analyses (step 3) were combined.