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. 2014 Jun 17;10:134. doi: 10.1186/1746-6148-10-134

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Copyright © 2014 Decorte et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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ELISA detection of PRRSV-specific IgA antibodies in reference standard samples. PRRSV-specific IgA in reference standard samples (kindly donated by prof. Zimmerman, College of Veterinary Medicine, Iowa State University) was measured with a modified PRRS X3 Ab Test (Idexx) as described by Kittawornrat et al. [12] that makes use of an HRP-conjugated goat-anti-pig IgA antibody (Bethyl Laboratories) PRRSV specific IgA in the reference samples was alternatively detected using the detection step of the IgA-specific IPMA, making use of biotinylated goat-anti-swine IgA antibodies (AbdSerotec) and Streptavidine-HRP.