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. 2014 Jun 25;3:10.3402/jev.v3.23784. doi: 10.3402/jev.v3.23784

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© 2014 Kerry L. Inder et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1 — Cavin-1 altered EV proteins and EV miR-148a with no effect on total cellular levels. (A) EVs and whole cell lysates (WCL) were collected from GFP-PC3 and cavin-1-PC3 cells and analysed by Western blot with the indicated antibodies. EV preparations were free from ER protein calnexin. Equal loading was shown by Coomassie staining. Images are representative of 3 biological replicates. Raw images of the western blots are available in figures in Supplementary file. (B) Cavin-1 alters the miRNA content of EVs. Real-time qPCR was performed on cDNA synthesized from equal amounts of RNA extracted from GFP-PC3-EVs and cavin-1-PC3-EVs or total cell lysates. Bar graphs show the mean relative expression±SEM of miR-148a was significantly decreased in cavin-1-PC3-EVs compared to GFP-PC3-EVs (p=0.02, n=3) but expression of miR-148a was unaffected in cells. (C) In contrast, miR-125a was not significantly different between GFP-PC3-EVs and cavin-1-PC3-EVs (n=3).