Fig. 1.

a A single-molecule kymograph of an individual mEos2 molecule, upon pulsed 405 nm irradiation (blue vertical lines). Taken from Annibale 2012. b The spectral evolution of partially photoconverted mEos2 upon 561 nm irradiation, displaying an increase in 405 nm absorbance, corresponding to the protonated form of the red fluorescent state. Taken from Annibale 2012. c The photoblinking phenomenon exists even at continuous activation. A typical kymograph of an mEos2 molecule embedded in a polymer gel, upon continous 405 nm irradiation at low intensity. Taken from Annibale et al. 2011. d A histogram of the number of times a single mEos2 molecule undergoes photoblinking (n _blink) before definitive photobleaching. Experimental values based on a single exponential best fit are shown, the 1/e decay values indicate a mean of n _blink = 1.05 ± 0.11. Taken from Annibale et al. 2011. e A histogram of the measured dark times showing a mean of t _off = 0.10 ± 0.01 s. Taken from Annibale et al. 2011