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Schematic of synthetic TMV. (A) Genome organization. (B) Long-oligonucleotides with 20 or 40 base overlaps. (C) Overlapping approximately 480 bp molecules made from long-oligonucleotides in (B). (D) Overlapping approximately 1,140 bp molecules made from (C). (E) Full-length DNA made from molecules from (D).
