Table 1. Photonic properties and pKa values for eGFP, bfloGFPa1 and bfloGFPc1.
eGFP | bfloGFPa1 | bfloGFPc1 | |
---|---|---|---|
Absorbance Maximum Peak (nm) | 488 | 497 | 493 |
FWHM (nm) | 45 | 55 | |
Fluorescence Maximum Excitation Peak (nm) | 488 | 500 | n.d. |
FWHM (nm) | 35–45 | 38 | |
Fluorescence Maximum Emission Peak (nm) | 508 | 512 | 521 |
FWHM (nm) | 30–40 | 62 | 58 |
Extinction Coefficient (M−1 cm−1) (per chain) | 56,60070 | 120,900 | 98,800 |
Quantum Yield (%) | 605 | 104 ± 5 | 0.15 ± 0.1 |
Brightness | 34 | 120.9 | 0.148 |
pKa | 5.65/5.95 | 3.0 | n.d. |
n.d.: not determined.