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. 2014 Jun 27;4:5469. doi: 10.1038/srep05469

Table 1. Photonic properties and pKa values for eGFP, bfloGFPa1 and bfloGFPc1.

  eGFP bfloGFPa1 bfloGFPc1
Absorbance Maximum Peak (nm) 488 497 493
FWHM (nm)   45 55
Fluorescence Maximum Excitation Peak (nm) 488 500 n.d.
FWHM (nm) 35–45 38  
Fluorescence Maximum Emission Peak (nm) 508 512 521
FWHM (nm) 30–40 62 58
Extinction Coefficient (M−1 cm−1) (per chain) 56,60070 120,900 98,800
Quantum Yield (%) 605 104 ± 5 0.15 ± 0.1
Brightness 34 120.9 0.148
pKa 5.65/5.95 3.0 n.d.

n.d.: not determined.