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. 2014 Jun 18;6(6):1882–1895. doi: 10.3390/toxins6061882

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Analysis of the recombinant PS5 protein expressed by E. coli BL21 (DE3) harboring the plasmid pET-33k. (A) Lane 1, uninduced soluble fraction (control). Lane 2, uninduced insoluble fraction (control). Lane 3, IPTG-induced soluble fraction. Lane 4, IPTG-induced insoluble fraction. Lane 5, alkali-solubilized insoluble 33-kDa protein; (B) Lane 1, alkali-solubilized insoluble 33-kDa protein purified by a nickel-chelating column. Lane 2, the protein of lane 1 treated with proteinase K. Lane 3, molecular mass standards.