Table 3.
Concentrations of phenolic acids and flavonoids of SBS extracts.
| Standards | RT 1 | λ 2 | Calibration curve | LOQ 3 | Compounds |
|---|---|---|---|---|---|
| Phenolic acids | |||||
| Phloroglucinol | 7.27 | 280 | Y = 397.949X + 0.655 | 50.00 | 0.60 ± 0.00 |
| 4-Hydroxy benzhydrazide derivative | 7.57 | 280 | Y = 8119.555X − 59.083 | 50.00 | 0.26 ± 0.00 |
| Gallic acid | 8.74 | 280 | Y = 18,200.182X − 28.003 | 50.00 | 0.53 ± 0.00 |
| Vanillic acid | 21.91 | 280 | Y = 11,026.185X + 14.026 | 50.00 | 1.44 ± 0.01 |
| Caffeic acid | 22.21 | 280 | Y = 19,697.774X − 13.018 | 50.00 | 0.74 ± 0.00 |
| Syringic acid | 24.10 | 280 | Y = 17,500.224X − 1.523 | 5.00 | 1.41 ± 0.01 |
| Chlorogenic acid | 24.92 | 280 | Y = 6240.064X − 10.524 | 50.00 | 1.61 ± 0.01 |
| p-Coumaric acid | 32.87 | 280 | Y = 23,926.358X + 0.631 | 5.00 | 10.41 ± 0.05 |
| trans-Ferulic acid | 34.48 | 280 | Y = 16,058.167X − 17.063 | 50.00 | 3.44 ± 0.02 |
| Sinapic acid | 34.91 | 280 | Y = 7025.930X + 0.785 | 50.00 | 2.98 ± 0.01 |
| 2-Amino-3,4-dimethyl-benzoicacid | 35.30 | 280 | Y = 1209.000X + 0.000 | 50.00 | 6.95 ± 0.04 |
| p-Anisic acid | 35.40 | 280 | Y = 9558.576X + 5.493 | 50.00 | - |
| Protocatechuic acid ethyl ester | 36.89 | 280 | Y = 8796.340X − 2.765 | 50.00 | 13.05 ± 0.06 |
| Coumarin | 38.27 | 280 | Y = 24,055.754X + 48.641 | 4.00 | 14.91 ± 0.04 |
| DPBA 4 | 39.85 | 280 | Y = 2971.415X − 7.872 | 50.00 | - |
| Alizarin | 43.86 | 280 | Y = 15,428.805X + 19.936 | 1.00 | - |
| Total phenolic acids | 62.98 ± 0.08 | ||||
| Flavonoids | |||||
| Gallocatechin | 17.68 | 280 | Y = 1331.637X + 0.000 | 50.00 | 1.59 ± 0.00 |
| Epigallocatechin | 18.58 | 280 | Y = 96.137X − 0.550 | 50.00 | 0.73 ± 0.00 |
| Catechin hydrate | 23.66 | 280 | Y = 3982.083X − 6.943 | 5.00 | 2.65 ± 0.01 |
| Epicatechin | 28.00 | 280 | Y = 7641.670X − 14.487 | 50.00 | 2.47 ± 0.00 |
| Epigallocatechin gallate | 29.53 | 280 | Y = 6425.894X − 6.592 | 50.00 | 0.98 ± 0.00 |
| Rutin hydrate | 32.93 | 370 | Y = 4763.242X − 4.752 | 50.00 | 0.29 ± 0.01 |
| Catechin gallate | 33.77 | 280 | Y = 1462.905X − 1.970 | 50.00 | - |
| Naringin | 34.14 | 280 | Y = 8230.457X − 42.997 | 50.00 | 2.60 ± 0.02 |
| Quercetin hydrate | 37.53 | 370 | Y = 7476.858X − 6.972 | 50.00 | 2.48 ± 0.02 |
| Myricetin | 37.41 | 370 | Y = 9908.955X − 0.383 | 5.00 | 0.43 ± 0.00 |
| Morin hydrate | 38.48 | 320 | Y = 4100.693X − 4.129 | 50.00 | - |
| Quercetin dehydrate | 40.19 | 370 | Y = 5623.574X − 0.729 | 50.00 | 1.93 ± 0.00 |
| Luteolin | 40.28 | 370 | Y = 12,303.249X − 8.820 | 50.00 | 6.32 ± 0.03 |
| Kaempferol | 42.89 | 370 | Y = 12,894.258X + 38.962 | 20.00 | 0.95 ± 0.03 |
| 3-Hydroxyflavone | 45.75 | 320 | Y = 4687.303X + 0.191 | 50.00 | - |
| Total flavonoids | 23.42 ± 0.09 |
Table 3 expressed by the RT, λ, calibration curve, LOQ and detected compounds. 1 RT, retention time; 2 λ, absorbance (nm); 3 LOQ, limit of quantitation; 4 DPBA, diphenylboric acid 2-aminoethyl ester. Data are presented as mean ± SD (n = 3).