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. 2014 Jun 27;4:5468. doi: 10.1038/srep05468

Figure 3. LPS-dependent Jun-N-terminal kinase activation is sensitive to space flight-associated microgravity.

Figure 3

The figure shows phospho-Jnk analysis of LPS stimulated monocytes in the presence (1 G) or relative absence of gravity (0 G). Fixed monocytes were stained fluorescently (green) and the nuclei were visualized by DAPI (blue) (Figure 3A). Comparison between individual cells clearly shows a brighter staining when stimulated with LPS in the presence of gravity. Staining intensity was measured at different exposure times (Figure 3B) and mean fluorescence was finally determined at 2000 ms (Figure 3D). Microgravity significantly impairs the activation of Jnk in monocytes. Analysis of nuclear localization of activated Jnk shows no change (Figure 3C). The graphs are based on 32 determinations. *P < 0.05.