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. 2014 Jun 5;11:104. doi: 10.1186/1743-422X-11-104

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Copyright © 2014 Huang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Detection of antibody responses by immunoblotting. Mice were immunized 1 ~ 4 times, at an interval of 2 weeks, with 100 μg plasmid DNAs. The serum samples were collected 13 days after the last immunization for titration of antibody responses by immunoblotting. Results are expressed as mean ± SD of 4 mice from each group. a. Not detectable.