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. 2014 Jun 19;15:23. doi: 10.1186/1471-2121-15-23

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Copyright © 2014 Kalb et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Analysis of TcCHC localization in Trypanosoma cruzi using a TcCHC monoclonal antibody. Subcellular localization of TcCHC in the various developmental forms of T. cruzi. (A-B): epimastigote. (C-D): metacyclic trypomastigotes. (E-F): isolated amastigote. Note strong immunolabeling in a region adjacent to the kinetoplast (arrow), where the flagellar pocket and Golgi complex are located. Nucleus (arrowhead) and kinetoplast DNA were stained with Hoechst 33342. A: rabbit anti-mouse IgG conjugated to Alexa Fluor 594 (pseudocolored in green); C &E: rabbit anti-mouse IgG conjugated to Alexa Fluor 488; B &F: Differential interference contrast (DIC) images of the parasite body; D: phase contrast images of the parasite body. Scale bars = 5 μm.