Table 1.
SP candidates of cT84.66 identified by nano-LC/LTQ/Orbitrap and then filtered with a set of criteria
| Peptides | Domain | Charge state |
Precursor m/z |
Top 5 most intensive product ions | Stabilitya | RT (min) | |
|---|---|---|---|---|---|---|---|
| During digestion | In autosampler | ||||||
| FSGTGSR | Light Chain | 2+ | 356.175 | 347.1; 477.2; 267.2; 235.1; 207.2 | Good | Poor | 26.6 |
| IDPANGNSK | Heavy Chain | 1+ | 915.453 | 687.5; 670.4; 898.5; 405.2; 519.4 | Poor | Poor | 31.9 |
| 2+ | 458.230 | 687.5; 344.5; 229.2; 255.1; 519.4 | 31.9 | ||||
| VDNALQSGNSQESVTEQDSK | Light Chain | 2+ | 1068.488 | 707.4; 1495.6; 893.3; 1051.7; 1902.9 | Poor | Good | 35.1 |
| 3+ | 712.661 | 748.6; 698.0; 812.6; 641.3; 606.2 | 35.1 | ||||
| ALPAPIEK | Heavy Chain | 1+ | 838.503 | 654.3; 486.3; 563.3; 340.2; 508.3 | Good | Good | 40.2 |
| VYACEVTHQGLR | Light Chain | 2+ | 716.854 | 586.1; 810.5; 667.6; 1170.6; 610.5 | Good | Good | 44.9 |
| 3+ | 478.239 | 586.1; 235.2; 668.2; 550.5; 263.3 | 44.9 | ||||
| DTLMISR | Heavy Chain | 1+ | 835.434 | 720.4; 461.2; 619.4; 506.3; 574.2 | Good | Poor | 47.6 |
| 2+ | 418.220 | 409.4; 506.3; 619.4; 375.2; 262.1 | 47.6 | ||||
| DTYMHWVK | Heavy Chain | 1+ | 1079.498 | 933.4; 1061.4; 834.4; 964.6; 648.2 | Good | Poor | 50.7 |
| 2+ | 540.252 | 531.4; 432.3; 482.7; 700.3; 863.6 | 50.7 | ||||
| STSGGTAALGCLVK | Heavy Chain | 1+ | 1264.657 | 1321.7; 1303.6; 576.6; 1133.7; 760.5 | Good | Good | 52.8 |
| 2+ | 632.832 | 652.4; 576.3; 689.5; 760.5; 831.4 | 52.8 | ||||
| ASNLESGIPVR | Light Chain | 1+ | 1142.616 | 628.4; 1124.6; 371.4; 541.4; 870.6 | Good | Good | 54.5 |
| 2+ | 571.812 | 371.4; 757.5; 772.3; 628.6; 541.5 | 54.5 | ||||
| NQVSLTCLVK | Heavy Chain | 2+ | 552.808 | 916.6; 820.5; 342.1; 519.5; 572.1 | Good | Good | 61.2 |
| LSCTASGFNIK | Heavy Chain | 1+ | 1197.593 | 1179.7; 1067.5; 1051.5; 665.5; 578.4 | Good | Good | 63.7 |
| 2+ | 599.301 | 499.6; 665.5; 837.5; 736.5; 578.4 | 63.7 | ||||
| FNWYVDGVEVHNAK | Heavy Chain | 1+ | 1677.801 | 1678.7; 853.4; 1659.8; 1531.6; 1460.7 | Good | Good | 65.5 |
| 2+ | 839.405 | 968.5; 853.3; 1067.5; 1230.6; 697.4 | 65.5 | ||||
| 3+ | 559.939 | 709.1; 534.6; 615.8; 485.0; 262.1 | 65.5 | ||||
| GPSVFPLAPSSK | Heavy Chain | 1+ | 1186.647 | 699.4; 1168.6; 751.4; 769.4; 846.5 | Good | Good | 69.3 |
| 2+ | 593.827 | 699.5; 846.4; 769.4; 418.4; 470.2 | 69.3 | ||||
| TPEVTCVVVDVSHEDPEVK | Heavy Chain | 2+ | 1041.507 | 1667.8; 472.2; 1039.5; 1154.5; 1253.6 | Good | Poor | 76.1 |
| 3+ | 694.673 | 834.6; 472.2; 971.2; 680.3; 856.8 | 76.1 | ||||
| QRPEQGLEWIGR | Heavy Chain | 2+ | 734.886 | 726.4; 531.4; 921.5; 1184.6; 345.2 | Good | Good | 81.4 |
| 3+ | 490.260 | 480.2; 612.2; 562.6; 660.4; 830.5 | 81.4 | ||||
| GFYPSDIAVEWESNGQPENNYK | Heavy Chain | 2+ | 1272.569 | 764.4; 1150.6; 1465.6; 1780.7; 1764.8 | Good | Poor | 84.7 |
| 3+ | 848.715 | 798.2; 1089.3; 950.5; 764.4; 733.5 | 84.7 | ||||
| VVSVLTVLHQDWLNGK | Heavy Chain | 2+ | 904.507 | 997.5; 1110.5; 896.7; 617.2; 1192.6 | Good | Good | 87.3 |
| 3+ | 603.340 | 806.1; 805.4; 712.6; 656.1; 499.5 | 87.3 | ||||
| TVAAPSVFIFPPSDEQLK | Light Chain | 2+ | 973.517 | 913.4; 802.7; 1015.5; 1060.5; 1320.6 | Good | Poor | 88.2 |
| 3+ | 649.347 | 457.5; 913.4; 873.5; 517.3; 1060.7 | 88.2 | ||||
| AGESVDIFGVGFLHWYQQK | Light Chain | 3+ | 727.697 | 869.7; 962.8; 919.2; 812.3; 575.6 | Poor | Poor | 95.0 |
a
peptide stabilities were evaluated by incubation in either digestion buffer or injection buffer (c.f. Experimental 2.5); Any candidate that degraded more than 20% was considered as "poor" stability.