Fig. 2.

Direct in vivo imaging of the functional effects of cellular heterogeneity. (A) Whole animal imaging of a KRAS(G12D)-induced RMS that was created in fluorescent transgenic zebrafish. myf5-GFP (green) labeled the cancer stem cell fraction, myogenein-H2-RFP (red; appears pink in this panel) was expressed in myoblast-like cells and mylz2-lynamCyan (blue) was expressed in terminally differentiated tumor cells. (B) Confocal image of the boxed region shown in panel A, showing the green, red and blue labels more clearly. (C) At later stages of RMS progression, cells became compartmentalized based on differentiation status, seen as clustering of labeled cells of the same color. (D,E) Higher-magnification views of the boxed regions from panel C, showing the regional compartmentalization of RMS cells based on differentiation status. Reproduced with permission from Ignatius et al. (Ignatius et al., 2012). Scale bars: 500 μm (A); 50 μm (B–E).