Fig. 4.

Recapitulating heterogeneity in vivo through cell transplantation. A heterogeneous T-ALL was created by mixing a single clone expressing dsRED, which had high leukemia-propagating potential and long latency, and a clone expressing GFP that had low tumor-propagating potential and short latency. Zebrafish were assessed for T-ALL growth at 15 and 45 days post-transplantation by whole-body fluorescence imaging (left) and confocal microscopy (right). Experiments revealed that proliferation and leukemia-propagating-cell frequency were regulated by different cellular processes, and that clones with high proliferation (green) outcompeted those with high leukemia-propagating frequency (red). Reproduced with permission from Blackburn et al. (Blackburn et al., 2014). Scale bars: 5 mm (left panels); 40 μm (right panels).