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. 2014 Jul;7(7):823–835. doi: 10.1242/dmm.014472

Fig. 2.

Fig. 2.

BFA, Tg and Tm differentially induce UPR sensors in the liver. (A,B) Standard PCR (A) and qPCR (B) analysis of xbp1 splicing in the livers of fish treated from 3 to 5 dpf with DMSO, 1 μg/ml BFA, 0.75 μM Tg or 1 μg/ml Tm using primers that amplify both unspliced (-u) and spliced (-s) xbp1. The average ratio of xbp1-second to xbp1-t is indicated (n=2). (C) Protein extracts from livers dissected from 5-dpf larvae treated as in A were blotted with anti-P-Eif2a and anti-tubulin as a loading control. Quantification and normalization to tubulin and DMSO controls is shown (n=2). (D) Analysis of atf4 and atf6 expression in livers of fish treated as in A. In B and D, target gene expression was normalized to rpp0 and fold changes compared to DMSO are plotted; n=13 for DMSO, 10 for Tm, 8 for Tg and 6 for BFA. Bars represent standard error in all graphs. *P<0.05, **P<0.01, calculated using paired Wilcoxon test.