Fig. 6.
Expression of genes and activation of pathways involved in pancreatic adenocarcinoma progression (real-time PCR data). Real-time PCR assay was used to confirm IHC data. (A,B) Increasing mRNA levels of TGFβ:mCherry reporter (A), cdh2 and nestin (B) were significant in Tg(ptf1a:Gal4;UAS:eGFP-KRASG12D) compared with the control Tg(ptf1a:eGFP. (C–E) Additional real-time PCR experiments revealed significantly unbalanced expression of several genes including tp53, TGFbR1b (C), notch1a, cyclinD1, her9 (D) and MYCn (E). Results are reported as mRNA level normalized to ྞ-actin. Error bars indicate s.e.m. for all analyzed data. Both Student’s t-tests and ANOVA tests were performed to statistically analyze data; *P<0.05, **P<0.01, ***P<0.005 comparing data between Tg(ptf1a:Gal4;UAS:eGFP-KRASG12D) and Tg(ptf1a:eGFP).