Figure 4. ESCRT-III polymer structure in transfected cells.
Anaglyphs of plasma membranes from COS-7 cells expressing (A) untagged CHMP4A, (B) CHMP4A(α1–α5), and (C–E) CHMP4A(α1–α5) and full-length CHMP2A together. (C) Cytoplasmic surface of the plasma membrane from an unroofed cell with high magnification shown in the inset, (D) corresponding top of a whole cell, and (E) top of a whole cell extracted with Triton X-100 and saponin after fixation to expose the underlying protein scaffold. Use view glasses for 3D structure (left eye = red). Scale bars represent 100 nm except in C where the scale bar on the survey view corresponds to 500 nm.