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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Neurochem Int. 2013 Sep 30;73:113–121. doi: 10.1016/j.neuint.2013.09.015

Figure 2.

Figure 2

Neuronal rictor deletion does not alter SERT expression in cortex and midbrain. Homogenized membranes were incubated with [3H]Citalopram [3.8 nM] for 45 minutes at room temperature. Non-specific binding was determined using unlabeled Fluoxetine [10 μM]. Data are expressed as mean ± SEM, n=3-10; p > 0.05 by Student’s t test. Inset: Neuronal rictor deletion in midbrain ablates pAkt-473, but not pAkt-308 or Akt protein, as demonstrated by immunoblotting. pAkt-473 values in both FLOX and rictor KO midrains were quantified and normalized to Akt. n=5/genotype, *p < 0.05 by Student’s t test.