Table 6. Morphological characteristics of cells from peripheral blood and spleen in ayu, Plecoglossus altivelis altivelis.

Part of collected sample blood cell	Cell type		Morphology	Cytoplasm	Nucleus
Caudal vein	A1 A2 Aa Ab	Thrombocyte Thrombocyte Thrombocyte Thrombocyte	A1:round,spindle A2:round,oval Aa:round or bean-shape Ab:spiked A1:aggregated A1,A2:pseudopodia	∙ A1:gray,light blue-purple ∙ A2:gray,light blue-purple, light blue ∙ Aa,Ab:colourless, gray or light blue-purple ∙ Ab: gray or light blue-purple	∙ A1:dark purplish-red, Aa:purplish-red, A2,Ab:light purplish-red ∙ A1:round or spindle, A2: round, kidney-shape or horseshoe-shape, Aa::round or bean-shape, Ab:rod-shape ∙ A1, A2:homogeneously packed chromatin ∙ Aa, Ab: faint rough condensed chromatin
Caudal vein	B1	Lymphocyte	B1:round, oval or bean-shapeB1:small cell	∙ Blue or light blue ∙ Golgi field	∙ Round or oval ∙ Rough condensed chromatin but indistinct chromatin net
Caudal vein	B2 B3	Lymphocyte Lymphocyte	B2,B3:round, oval B2,B3middle,large cell B2,B3:pseudopodia	∙ Blue-gray or light blue ∙ Colorless vesicles ∙ Golgi field	∙ Kidney-shape, horseshoe-shape, or infinite-shape ∙ Rough condensed chromatin.
Caudal vein	C1 C2 C3	Neutrophil Neutrophil Neutrophil	∙ Round or oval ∙ Small~large cell	∙ Light blue gray or light peach gray ∙ Invisible granules	∙ Round, spindle, kidney-shape or rod-shape ∙ Bilobed nuclei ∙ Rough condensed chromatin ∙ C1:faint rough condensed chromatin
Caudal vein	D	Basophil	∙ Oval	∙ Light blue-gray and light red in part ∙ Large blue-purple granules	∙ Kidney-shape ∙ Large blue-purple granules on nucleus ∙ Unclear chromatin net
Caudal vein	E	Monocyte/ Macrophage	∙ Large cell ∙ Oval ∙ Pseudopodia	∙ Light blue gray ∙ Blue granules, dark red-purple stained inclusions and vesicles/vacuole ∙ Invisible Golgi-like field	∙ Kidney-shape ∙ Lineal packed chromatin
Spleen	F	Monocyte/ Macrophage	∙ Large cell ∙ Round, oval or amoeboid-shape ∙ Pseudopodia	∙ Light blue gray ∙ Black-blue or black-brown stained inclusions and vesicles/vacuoles ∙ Invisible Golgi field	∙ Oval, kidney, rod or amorphous ∙ Lineal packed chromatin but indistinct chromatin net