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. 2014 Feb 7;25(7):1486–1495. doi: 10.1681/ASN.2013070773

Figure 3.

Figure 3.

Knockdown of Gal-1 downregulated CXCR4. (A) The effects of Gal-1 silencing on cancer-associated genes expression were detected by RT-qPCR. (B) Validation of CXCR4 protein expression after Gal-1 knockdown was demonstrated by Western blotting (WB). (C) CXCR4 was silenced by the lentiviral shCXCR4 infection in A-498 cells. Both mRNA and protein levels of CXCR4 were detected by RT-PCR and Western blotting, respectively. (D) The effects of CXCR4 knockdown on cell invasion (upper panel) and migration (lower panel) were examined in A-498 cells. Relative invasion or migration ability was normalized to that of cells transduced with shLuc. (E) The colony-forming ability of CXCR4-infected A-498 cells was evaluated by clonogenic (upper panel) and soft agar assays (lower panel). Results are representative of three independent experiments. Data are mean±SD. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. **P<0.01; ***P<0.001.