Table 2.
Type of mRNA | ACR (copies/μg total RNA, median [IQR]; n=26) | AMR (copies/μg total RNA, median [IQR]; n=26) | ATI (copies/μg total RNA, median [IQR]; n=32) | P Value (Kruskal–Wallis Test) | P Value (Dunn’s Test) | ||
---|---|---|---|---|---|---|---|
ACR Versus AMR | ACR Versus ATI | AMR Versus ATI | |||||
CD3ε | 27,350 (15,525–96,400) | 2690 (875–8540) | 382 (108–1415) | <0.001 | <0.05 | <0.05 | <0.05 |
Granzyme B | 17,400 (4105–33,950) | 2240 (708–5610) | 444 (190–2105) | <0.001 | <0.05 | <0.05 | >0.05 |
Perforin | 12,950 (4745–48,125) | 1920 (407–6443) | 21 (84–1313) | <0.001 | <0.05 | <0.05 | <0.05 |
FoxP3 | 541 (371–1080) | 119 (13–288) | 12.5 (12.5–12.5) | <0.001 | <0.05 | <0.05 | <0.05 |
OX40 | 4885 (1007–11,450) | 422 (127–2033) | 235 (12.5–385) | <0.001 | <0.05 | <0.05 | >0.05 |
CD105 | 32,550 (13,750–55,750) | 6890 (1785–16,450) | 2950 (663–8398) | <0.001 | <0.05 | <0.05 | >0.05 |
CD146 | 983 (630–2923) | 573 (126–1323) | 316 (56–750) | <0.01 | >0.05 | <0.05 | >0.05 |
von Willebrand factor | 1245 (523–2680) | 718 (121–1960) | 316 (88–1096) | <0.01 | >0.05 | <0.05 | >0.05 |
IgJ | 1210 (571–4525) | 523 (149–3660) | 72 (12.5–192) | <0.001 | >0.05 | <0.05 | <0.05 |
PSMB10 | 214,500 (66,525–484,500) | 28,900 (9345–58,975) | 28,550 (6253–44,150) | <0.001 | <0.05 | <0.05 | >0.05 |
TRIB1 | 227,000 (145,250–452,750) | 62,450 (18,900–176,500) | 86,200 (18,750–242,500) | <0.01 | <0.05 | <0.05 | >0.05 |
TLR4 | 25,200 (5363–67,525) | 6800 (975–19,725) | 4140 (444–13,775) | <0.01 | <0.05 | <0.05 | >0.05 |
CD14 | 290,500 (117,025–699,250) | 21,500 (9548–88,500) | 55,950 (3083–182,250) | <0.001 | <0.05 | <0.05 | >0.05 |
Complement factor 3 | 16,700 (6028–43,550) | 6445 (1233–13,950) | 2120 (745–8318) | <0.001 | <0.05 | <0.05 | >0.05 |
Complement factor 5 | 806 (374–1733) | 260 (46–535) | 97 (12.5–390) | <0.001 | <0.05 | <0.05 | >0.05 |
Properdin | 68,500 (19,600–208,500) | 8960 (3165–47,425) | 21,450 (1858–59,400) | <0.01 | <0.05 | <0.05 | >0.05 |
Complement factor B | 31,900 (14,200–58,925) | 27,150 (14,150–51,475) | 15,250 (6085–26,575) | 0.01 | >0.05 | <0.05 | >0.05 |
CD55 | 571,000 (321,500–1,403,000) | 175,500 (63,075–530,000) | 173,500 (44,475–891,750) | <0.01 | <0.05 | <0.05 | >0.05 |
CD46 | 749,500 (328,000–1,438,000) | 173,500 (83,100–411,750) | 272,000 (60,075–526,500) | <0.001 | <0.05 | <0.05 | >0.05 |
Vimentin | 1,430,000 (556,000–3,810,000) | 390,000 (91,100–800,500) | 295,500 (53,350–844,250) | <0.001 | <0.05 | <0.05 | >0.05 |
NKCC2 | 3130 (967–7133) | 1031 (214–3480) | 1110 (191–2863) | <0.01 | <0.05 | <0.05 | >0.05 |
E-cadherin | 19,200 (7248–41,700) | 7160 (3443–15,675) | 5310 (1246–14,675) | <0.01 | <0.05 | <0.05 | >0.05 |
IL-6 | 1307 (576–10,433) | 328 (47–2598) | 151 (12.5–1935) | <0.01 | <0.05 | <0.05 | >0.05 |
CXCL13 | 7920 (3428–50,600) | 437 (12.5–3865) | 78 (12.5–459) | <0.001 | <0.05 | <0.05 | >0.05 |
CD20 | 3360 (644–6805) | 449 (133–2088) | 89 (12.5–403) | <0.001 | <0.05 | <0.05 | <0.05 |
TGFβ1 | 117,000 (44,350–207,250) | 27,950 (5410–68,750) | 18,050 (2978–46,675) | <0.001 | <0.05 | <0.05 | >0.05 |
18S rRNA | 1.12×1010 (6.68×109–3.62×1010) | 4.15×109 (1.97×109–9.45×109) | 4.28×109 (1.40×109–1.19×1010) | <0.01 | <0.05 | <0.05 | >0.05 |
The 26 mRNAs and 18S rRNA were quantified using gene-specific primers and probes by real-time PCR assay. Median absolute copy numbers per microgram of total RNA (IQR; interquartile range) of each mRNA measure and 18S rRNA are shown. P values were calculated using Kruskal–Wallis test of no differences among the ACR, AMR, and ATI biopsy groups. After Kruskal–Wallis test, pairwise comparisons among the three groups were done using the Dunn’s test. P values<0.05 were considered statistically significant. The rationale for the mRNAs selected for inclusion in the mRNA panel is provided in the Supplemental Appendix.