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. Author manuscript; available in PMC: 2014 Jun 27.
Published in final edited form as: J Org Chem. 2011 Apr 29;76(10):3853–3860. doi: 10.1021/jo200265u

Figure 4.

Figure 4

(A) EPR spectrum of the solution containing the trityl and nitroxide monoradicals under anaerobic conditions; spectra were recorded with 0.5 mW microwave power, 0.03 G modulation amplitude and 50 G sweep width. (B) EPR spectrum of the sample in (A) with a narrow sweep width (1.4 G) to better visualize the triplet hyperfine structure of the trityl monoradical. (C) Plot of Iin/Iout for the trityl monoradical(s) as a function of O2%. The trityl monoradical were generated by mixing the biradical TSSN (500 μM) with GSH (3 mM) in NaOH (10 mM) for 60 min since GSH has a higher reactivity towards the disulfide bond at high pH as mentioned previously;40 The resulting solution was diluted 5-fold using PB (0.1 M, pH 7.4) and used for analysis of oxygen sensitivity.