Fig. 2.

AMH induces vascular formation and alters epithelial morphology in XX gonads. 11.5 dpc gonads were cultured for 40 h in the presence or absence of 15 μg/ml AMH. Immunostaining was performed for PECAM-1 (Pharmingen), which labels germ cells (round, red) and vascular endothelial cells (elongated, red), and for laminin (H. Erickson, Duke University), which labels the basal lamina underlying the coelomic epithelium (green). (A, and B) Cultured XY gonads display a characteristic vessel beneath the coelomic epithelium (small arrows), and a thickened coelomic epithelium containing multiple cell layers (large arrowheads). (C, and D) These features are absent in XX gonads. (E, and F) XX gonads cultured with AMH contain vessels extending underneath the epithelial layer (small arrows) and a epithelial morphology more similar to that observed in XY controls (large arrowheads). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)