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. Author manuscript; available in PMC: 2014 Jun 27.
Published in final edited form as: Mol Cell Endocrinol. 2003 Dec 15;211(0):1–7. doi: 10.1016/j.mce.2003.09.021

Fig. 3.

Fig. 3

AMH does not induce expression of Sertoli or Leydig cell markers in XX gonads. Gonads and mesonephroi from 11.5 dpc embryos were cultured for 48 h in the absence or presence of 15 μg/ml AMH and whole mount in situ hybridization for Sox9 or Scc mRNA was performed as previously described (Tilmann and Capel, 1999). (A) Sox9 expression was observed in XY gonads (top), but was absent in XX gonads cultured under similar conditions (middle). XX gonads cultured in the presence of AMH (bottom) also did not express detectable levels of Sox9. (B) Scc expression was also observed in XY controls (top) but was not induced in AMH-treated XX gonads (bottom).