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Isolation and Partial Purification of purine nucleoside phosphorylase (PNP) from S. mansoni by the use of chromatofocussing column chromatography and molecular sieving
| Fraction | Total volume (ml) | Total protein | Total units (nmol/min) | Specific Activitya (nmol/min/mg protein) | Yield % |
|---|---|---|---|---|---|
| Cytosol | 7.7 | 88.9 | 73 | 0.82 | 100 |
| Chromatofocussing | 20 | 14.2 | 118 | 8.2 | 162 |
| Molecular sieving | 1.5 | 0.84 | 28 | 34.0 | 38 |
