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. 2014 Jun 27;8:68. doi: 10.3389/fncir.2014.00068

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Copyright © 2014 Hayworth, Morgan, Schalek, Berger, Hildebrand and Lichtman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cerebellum UTSL example data. (A) Image of WaferMapper GUI showing a graphically-defined 3 × 5-tile montage targeted near the surface of a cerebellar folium. Zoom overlays show high resolution images of that region. (B) Montage images of three successive ultrathin sections in this cerebellum UTSL acquired automatically by WaferMapper (scale bar = 100 μm). Red arrow shows location of corresponding high resolution images shown in (C). (C) Cut outs of high resolution data imaged at 10 MPS and aligned in FIJI (scale bar = 1 μm). Asterisks mark position of two synapses.