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. Author manuscript; available in PMC: 2014 Jun 27.
Published in final edited form as: J Surg Res. 2013 Dec 18;187(1):6–13. doi: 10.1016/j.jss.2013.12.016

Fig. 1.

Fig. 1

NSCLC cell lines show variable CHK1 expression. (A) Microarray analysis of CHK1 mRNA levels in 79 lung cancer cell lines. The microarray data were downloaded from Gene Expression Omnibus using the accession number GSE4824 and robust multiarray average (RMA) normalized. P53 mutation information was acquired from http://p53.free.fr/index.html. (B) Relative expression of CHK1 mRNA to β-actin by qRT-PCR in selected four cell lines, H23, H1437, H1993, and H1299. One representative result is shown (C) CHK1 expression by immunohistochemistry of the cell lines showing increased nuclear CHK1 protein expression (brown stain) in the H1299, H23, and H1437 high CHK1 expressing cells as compared with H1993 cells low CHK1 expressing cells. (Color version of figure is available online.)