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. Author manuscript; available in PMC: 2014 Jun 27.
Published in final edited form as: Nature. 2013 Dec 12;504(7479):315–318. doi: 10.1038/nature12832

Extended Data Figure 2. ATP indirectly activates the cilia conductance from four different cell types.

Extended Data Figure 2

Top, Single channel currents activated by 1.5 s depolarizations to the indicated potentials in control (black traces) and 100 μM extracellular ATP (red traces) recorded from primary cilia derived from (a) human RPE Smo-GFP cell lines, (b) mouse RPE Arl-GFP primary cells, (c) mouse MEF Arl-GFP primary cells, (d) mouse kidney IMCD Arl-EGFP cells (scale = 10 pA and 200 ms). Bottom, corresponding open probability histograms measured in control (grey) and in the presence of 100 μM ATP (red; ± SEM, n = 4-6 cilia, asterisks indicates P < 0.005). (e) Average open dwell times measured from the cilia of these four cell types in control and ATP conditions.