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. Author manuscript; available in PMC: 2014 Jun 27.
Published in final edited form as: Nature. 2013 Dec 12;504(7479):315–318. doi: 10.1038/nature12832

Extended Data Figure 3. Anti-PKD1-L1 and -PKD2-L1 siRNA treatment attenuates the RPE ciliary current.

Extended Data Figure 3

(a) Table of primers used to detect transcript levels present in human RPE cells. (b) Table of siRNAs and their knockdown efficiencies used to identify channel candidates. (c) Example ciliary current measured from cells treated with siRNAs specific for PKD1-L1 or PKD2-L1. (c) Box (± SEM) and whisker (± SD) plots of cilia total outward (+100 mV) and inward (-100 mV) current measured 72 h after double-siRNA treatment. PKD-L mRNAs were targeted by two siRNAs specific for two different regions of the target transcript. Averages are indicated by the red lines. Student's t-test P values comparing treatment groups to scrambled siRNA: * denotes P-value < 0.05; n = 8-12 cilia.