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Imbalance of soluble and insoluble gamma-tubulin in a panel of human breast tumor cell lines with increasing metastatic potential. Western blot analysis was performed on whole cell lysates after ultracentrifugation to separate soluble and insoluble fractions (A). Bands were analyzed by densitometry measurements using ImageJ and graphed as ratios (B). Soluble/insoluble ratios of MDA-MB231 and HCC1937 are significantly different (denoted by *) from MCF-10A (p<0.05).
