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. Author manuscript; available in PMC: 2014 Jun 27.
Published in final edited form as: Cancer Biol Ther. 2010 Jan 28;9(1):66–76. doi: 10.4161/cbt.9.1.10451

Figure 6.

Figure 6

Immunofluorescence with prefixation permeabilization reveals gamma-tubulin is incorporated in microtubules. Cells treated with colchicine provide evidence of gamma-tubulin (A-D and I-L) along microtubules in breast cancer cell lines (panel B, red arrow), while a normal centrosome staining for non-tumorigenic MCF-10A. N-P vs. F-H show a stabilization effect of alpha-tubulin (green stain) in breast cancer cell lines when treated with colchicine. HCC1937 shows short pieces of microtubules when treated with colchicine (panel P, white arrow), which indicates stabilization of fragments of alpha-tubulin by gamma-tubulin. HCC1937 also shows membrane staining of gamma-tubulin without colchicine treatment (panel D, white arrowhead), but this staining pattern is lost with colchicine treatment (panel P).