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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Mol Cell Cardiol. 2014 Apr 26;72:350–359. doi: 10.1016/j.yjmcc.2014.04.010

Figure 3. NRVMs rapidly conduct dATP-fluorescein between cells with kinetics similar to fluorescein alone.

Figure 3

(A) NRVM cultures were microinjected with dATP-fluorescein or fluorescein and serially imaged for 5 minutes. Images were thresholded and quantified for (B) maximum area of dye diffusion and (C) maximum diffusion distance from the pipette tip. To assess gap junction specificity, we also added 2mM heptanol and observed a significant decrease in transfer efficiency. n=3–9 per condition. *p<0.05