Table 1.
Quantification and quality assurance of RNA using the NanoDrop ND-1000
| Sample ID | OD260/280 Ratio* | OD260/230 Ratio* | Conc. (ng/uL) | Volume (uL) | Quantity (ng) | QC result (Pass/Fail) |
|---|---|---|---|---|---|---|
| B93C | 1.88 | 2.04 | 352.40 | 10 | 3524.00 | Pass |
| B95C | 1.88 | 2.03 | 254.01 | 10 | 2540.10 | Pass |
| B98C | 1.90 | 2.08 | 620.42 | 10 | 6204.20 | Pass |
| B93T | 2.02 | 2.03 | 1257.93 | 10 | 75475.80 | Pass |
| B95T | 1.84 | 1.97 | 127.72 | 10 | 1277.20 | Pass |
| B98T | 1.93 | 1.98 | 521.22 | 10 | 20848.80 | Pass |
*
The OD260/280 ratio measured with a spectrophotometer should be closer to 2.0 for pure RNA (ratios between 1.8 and 2.1 are acceptable).
The OD260/230 ratio should be higher than 1.8.