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. Author manuscript; available in PMC: 2014 Jun 27.
Published in final edited form as: Methods Mol Biol. 2014;1062:629–658. doi: 10.1007/978-1-62703-580-4_33

Figure 1.

Figure 1

Different examples for BiFC applications. BiFC can be applied to investigate (a) interactions of single PPI pairs (BiFC), (b) co-localization of different PPI pairs (coBiFC), (c) competition of two protein complex formations (mcBiFC), (d) analyses of ternary protein complex formations (BiFC based FRET or BRET) or (e) the analysis of quaternary protein complexes (by combining BiLC and BiFC). The protein fragment and/or donor/acceptor combinations recommended for each application and the resulting excitation and emission maxima or substrate catalysis of the respective (complemented) proteins are indicated. The proteins to be investigated for PPI are labeled from A – D. For more information refer to main text.