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. 2014 Feb 7;210(2):200–208. doi: 10.1093/infdis/jiu085

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© The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Gel and Western blotting analysis of purified χE559^P. Purified, plant-derived chimeric E559 (χE559^P) was analyzed by Coomassie staining of polyacrylamide gels under nonreducing (panel A) and reducing (panel B) conditions, and by Western blotting under nonreducing (panels C and E) and reducing (panels D and F) conditions. For Western blotting, the nitrocellulose membranes were probed with HRP-conjugated antibodies specific for heavy chains (panels C and D), or with HRP-conjugated antibodies specific for light chains (panels E and F). The χE559^P samples (lane 1) were probed with human-specific reagents, whereas E559^Hyb samples (lane 2) were probed with murine-specific reagents. Abbreviations: HRP, horseradish peroxidase; M, molecular weight standards. Asterisks indicate the positions of the fully assembled antibodies.