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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: Immunity. 2014 May 1;40(5):681–691. doi: 10.1016/j.immuni.2014.04.006

Figure 4. Antigen Stimulation Downmodulates p53 Expression Induced by IL-2.

Figure 4

(A) WT clones were stimulated for 48 hr (top) and for the indicated times up to 96 hr (bottom), and p53 protein was analyzed by immunoblotting. β-actin was used as internal control. The data are representative of three independent experiments.

(B) WT and Trp53−/− clones were stimulated as indicated for 96 hr and p53 protein expression was analyzed by flow cytometry. Trp53−/− clones were used as a negative control for p53 staining. Results are representative of three independent experiments.

(C) Statistical analysis of the results shown in (B). The graph presents means ± SEM of three clones each of WT and Trp53−/− and representative of three independent experiments.