Skip to main content
. 2014 Jun 5;3(7):787–791. doi: 10.5966/sctm.2013-0036

Figure 1.

Figure 1.

hiPSC generation from keratinocytes using episomal vectors. (A): Schematic timeline. (B): Cell morphology and GFP expression of keratinocytes during reprogramming at days 1, 8, 12, and 19 using six reprogramming factors (MKOSLsh) and GFP. Scale bar = 100 μm. (C): Representative morphology of a non-ESC-like colony and an ESC-like colony reprogrammed from keratinocytes. Scale bar = 100 μm. (D): Representative image of AP staining of FiPSCs and KiPSCs in a 100-mm dish. (E): Quantification of FiPSCs or KiPSCs using four reprogramming factors (KOSsh) or six reprogramming factors (MKOSLsh). ∗∗∗ = p < .05, unpaired two-tailed t test. Abbreviations: AP, alkaline phosphatase; bFGF, basic fibroblast growth factor; ESC-like, embryonic stem cell-like; FiPSC, hiPSC colonies generated from fibroblasts; GFP, green fluorescent protein; hESC-like, human embryonic stem cell-like; hiPSC, human induced pluripotent stem cell; KiPSC, hiPSC colonies generated from keratinocytes; KOSsh, KLF4, OCT4, SOX2, and short hairpin RNA for p53; KSR, knockout serum replacement; MEF, mouse embryonic fibroblast; MKOSLsh, L-MYC, KLF4, OCT4, SOX2, LIN28, and short hairpin RNA for p53.