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Wnt-responsive cells are not colocalized with the HIF1α-positive cells in the p53-null mammary tumors. (A): DAPI staining. (B): HIF1α-positive regions (circled) and representative HIF1α-positive cells (red arrows). (C): eGFP-positive Wnt-responsive cells (none was observed within this hypoxic region). (D): Merged images. Scale bars = 50 μm. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; eGFP, enhanced green fluorescent protein; HIF, hypoxia-inducible factor.
